1,202 research outputs found
La recherche juridique sur les prélèvements biologiques réalisés dans le cadre des autopsies et objets de scellés judiciaires. Nécessité d'une adaptation législative
Currently, it is legally impossible to conduct scientific research on tissue and organ samples taken from forensic autopsies. In fact, the law schedules the destruction of such samples at the end of the judicial investigation, and the common law rules governing cadaver research cannot be applied to the forensic context. However, nothing seems in itself to stand in the way of such research since, despite their specific nature, these samples from forensic autopsies could be subject, following legislative amendments, to common law relating to medical research on samples taken from deceased persons. But an essential legislative amendment will have the goal firstly to allow the Biomedicine Agency to become authorized to issue a research permit and secondly, to change the research conditions in terms of the non-opposition of the deceased to the said research. Such an amendment would be a true breakthrough because it would allow teams to continue to move forward calmly in research, and allow this research to be placed within a legal framework, which would promote international exchanges
Full-genome sequence of the plant growth-promoting bacterium Pseudomonas protegens CHA0.
We report the complete genome sequence of the free-living bacterium Pseudomonas protegens (formerly Pseudomonas fluorescens) CHA0, a model organism used in plant-microbe interactions, biological control of phytopathogens, and bacterial genetics
Rapid evolution of trait correlation networks during bacterial adaptation to the rhizosphere
There is a growing awareness that traits do not evolve individually but rather are organized as modular networks of covarying traits. Although the importance of multi-trait correlation has been linked to the ability to evolve in response to new environmental conditions, the evolvability of the network itself has to date rarely been assessed experimentally. By following the evolutionary dynamics of a model bacterium adapting to plant roots, we demonstrate that the whole structure of the trait correlation network is highly dynamic. We experimentally evolved Pseudomonas protegens, a common rhizosphere dweller, on the roots of Arabidopsis thaliana. We collected bacteria at regular intervals and determined a range of traits linked to growth, stress resistance, and biotic interactions. We observed a rapid disintegration of the original trait correlation network. Ancestral populations showed a modular network, with the traits linked to resource use and stress resistance forming two largely independent modules. This network rapidly was restructured during adaptation, with a loss of the stress resistance module and the appearance of new modules out of previously disconnected traits. These results show that evolutionary dynamics can involve a deep restructuring of phenotypic trait organization, pointing to the emergence of novel life history strategies not represented in the ancestral phenotype
Preface: Approaches and methods to improve risk management in volcanic areas
Active volcanoes can generate multiple types of
geological hazards. Besides syneruptive threats (e.g., lava,
pyroclastic flows or ash fall), other adverse events such as
landslides or lahars can occur at any time. To manage these
threats efficiently, three key objectives must be jointly addressed:
(1) improving prevention tools, through the collection
and acquisition of data on hazards and risks, and
its dissemination as maps and scenarios; (2) improving crisis
management capabilities, based on monitoring and early
warning systems, but also reliable communications systems;
and (3) reducing people’s vulnerability and developing recovery
and resilience capabilities after an event has occurred.
The special issue “Approaches and methods to improve
risk management in volcanic areas” presents research
results focusing on these three objectives. It demonstrates
the utility of addressing them jointly, and particularly examines
the case of volcanoes where little knowledge is available.
These results were presented at the conference Integrated
Approaches for Volcanic Risk Management (Hohenheim
University, Stuttgart, 11/12 September 2012) of the European
MIAVITA (MItigate and Assess risk from Volcanic
Impact on Terrain and human Activities) project
Selection rules in three-body B decay from factorization
Extending the dynamics underlying the factorization calculation of two-body
decays, we propose simple selection rules for nonresonant three-body B decays.
We predict, for instance, that in the Dalitz plot of B^0-->D^0-bar\pi^+\pi^-,
practically no events should be found in the corner of E(\pi^+) < \Lambda_{QCD}
as compared with the corner of E(\pi^-) < \Lambda_{QCD}. We also predict that
there should be very few three-body decay events with a soft meson resonance
and two energetic mesons or meson resonances. The selection rules are quite
different from the soft pion theorem, since they apply to different kinematical
regions. For B^0 -->D^0-bar\pi^+\pi^-, the latter predicts that the decay
matrix element vanishes in the zero-four-momentum limit of \pi^+ instead of
\pi^-. Since this marked difference from the soft pion theorem is directly
related to the issue of short-distance QCD dominance in the FSI of two-body B
decays, experimental test of the selection rules will shed light on strong
interaction dynamics of B decay.Comment: 12 pages in REVTEX including 3 eps figure
Pseudomonas fluorescens CHA0 maintains carbon delivery to Fusarium graminearum-infected roots and prevents reduction in biomass of barley shoots through systemic interactions
Soil bacteria such as pseudomonads may reduce pathogen pressure for plants, both by activating plant defence mechanisms and by inhibiting pathogens directly due to the production of antibiotics. These effects are hard to distinguish under field conditions, impairing estimations of their relative contributions to plant health. A split-root system was set up with barley to quantify systemic and local effects of pre-inoculation with Pseudomonas fluorescens on the subsequent infection process by the fungal pathogen Fusarium graminearum. One root half was inoculated with F. graminearum in combination with P. fluorescens strain CHA0 or its isogenic antibiotic-deficient mutant CHA19. Bacteria were inoculated either together with the fungal pathogen or in separate halves of the root system to separate local and systemic effects. The short-term plant response to fungal infection was followed by using the short-lived isotopic tracer 11CO2 to track the delivery of recent photoassimilates to each root half. In the absence of bacteria, fungal infection diverted carbon from the shoot to healthy roots, rather than to infected roots, although the overall partitioning from the shoot to the entire root system was not modified. Both local and systemic pre-inoculation with P. fluorescens CHA0 prevented the diversion of carbon as well as preventing a reduction in plant biomass in response to F. graminearum infection, whereas the non-antibiotic-producing mutant CHA19 lacked this ability. The results suggest that the activation of plant defences is a central feature of biocontrol bacteria which may even surpass the effects of direct pathogen inhibition
On the Mixing of the Scalar Mesons , and
Based on a mass matrix describing the mixing of the scalar states
, and , the hadronic decays of the three
states are investigated. Taking into account the two possible assumptions
concerning the mass level order of the bare states
, and in the
scalar sector, and , we obtain the
glueball-quarkonia content of the three states by solving the unlinear
equations. Some predictions about the decays of the three states in two cases
are presented, which can provide a stringent consistency check of the two
assumptions.Comment: revtex 10 pages, 1 eps figur
Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10
The diffusion of KPC-2 carbapenemase is closely related to the spread of Klebsiella pneumoniae of the clonal-group 258 and linked to IncFIIK plasmids. Little is known about the biology of multi-drug resistant plasmids and the reasons of their successful dissemination. Using E. coli TOP10 strain harboring a multi-replicon IncFIIK-IncFIB blaKPC−2-gene carrying plasmid pBIC1a from K. pneumoniae ST-258 clinical isolate BIC-1, we aimed to identify basal gene expression and the effects of imipenem exposure using whole transcriptome approach by RNA sequencing (RNA-Seq). Independently of the antibiotic pressure, most of the plasmid-backbone genes were expressed at low levels. The most expressed pBIC1a genes were involved in antibiotic resistance (blaKPC−2, blaTEM and aph(3′)-I), in plasmid replication and conjugation, or associated to mobile elements. After antibiotic exposure, 34% of E. coli (pBIC1a) genome was differentially expressed. Induction of oxidative stress response was evidenced, with numerous upregulated genes of the SoxRS/OxyR oxydative stress regulons, the Fur regulon (for iron uptake machinery), and IscR regulon (for iron sulfur cluster synthesis). Nine genes carried by pBIC1a were up-regulated, including the murein DD-endopeptidase mepM and the copper resistance operon. Despite the presence of a carbapenemase, we observed a major impact on E. coli (pBIC1a) whole transcriptome after imipenem exposure, but no effect on the level of transcription of antimicrobial resistance genes. We describe adaptive responses of E. coli to imipenem-induced stress, and identified plasmid-encoded genes that could be involved in resistance to stressful environments
Electromagnetic form factors in the J/\psi mass region: The case in favor of additional resonances
Using the results of our recent analysis of e^+e^- annihilation, we plot the
curves for the diagonal and transition form factors of light hadrons in the
time-like region up to the production threshold of an open charm quantum
number. The comparison with existing data on the decays of J/\psi into such
hadrons shows that some new resonance structures may be present in the mass
range between 2 GeVand the J/\psi mass. Searching them may help in a better
understanding of the mass spectrum in both the simple and a more sophisticated
quark models, and in revealing the details of the three-gluon mechanism of the
OZI rule breaking in K\bar K channel.Comment: Formulas are added, typo is corrected, the text is rearranged.
Replaced to match the version accepted in Phys Rev
Possible large phase in psi(2S) -> 1-0- Decays
The strong and the electromagnetic amplitudes are analyzed on the basis of
the measurements of J/psi, psi(2S) -> 1-0- in e+e- experiments. The currently
available experimental information is revised with inclusion of the
contribution from e+e- -> gamma * -> 1-0- . The study shows that a large phase
around minus 90 degree between the strong and the electromagnetic amplitudes
could not be ruled out by the experimental data for psi(2S).Comment: 4 page
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